中国药理学通报Issue(1)：114-118,5.DOI:10.3969/j.issn.1001-1978.2016.01.024
高糖对肾小管细胞胆固醇转运的影响及花青素的干预研究
Effect of high glucose on cholesterol efflux in renal tubular cell and intervention of anthocyanins
摘要
Abstract
Aim To investigate the effects of high glu-cose on cholesterol metabolism in renal tubular cells and the intervention of the anthocyanins. Methods HK-2 cells were grown in the DMEM medium supple-mented with 10% FBS and were divided into 5 groups:normal glucose group, high glucose group, mannitol group, C3G group and Cy group. Effect of anthocya-nins on cell viability was detected with MTT, and cho-lesterol accumulation was detected with Amplex Red Cholesterol Assay kit and Filipin staining. Expression of ABCA1 was detected with RT-qPCR and Western Blot. Results In compared with control groups, HG significantly promoted cholesterol mass inside the cell and decreased the cholesterol concentration in the me-dium after treatment for 24 h or 48 h. The levels of mRNA and protein of ABCA1 were detected with RT-qPCR and Western blot, and both were decreased in the presence of HG. Whereas treatment with C3G and Cy markedly attenuated HG-induced cholesterol mass inside the cell by up-regulating the expression of AB-CA1. Conclusions High glucose can reduce the ex-pressions of the ABCA1, and then decrease cholesterol efflux and increase the cholesterol accumulation in HK-2 cells. Anthocyanins can decrease cholesterol accu-mulation by up-regulating the expression of ABCA1.关键词
花青素/高糖/肾小管上皮细胞/ABCA1/胆固醇/糖尿病肾病Key words
anthocyanins/high glucose/tubular epi-thelial cells/ABCA1/cholesterol/diabetic nephropathy分类
医药卫生引用本文复制引用
杜春阳,史永红,朱艳,任韫卓,吴海江,韦金英,吴明,肖夏,段惠军..高糖对肾小管细胞胆固醇转运的影响及花青素的干预研究[J].中国药理学通报,2016,(1):114-118,5.基金项目
河北省卫生厅重点科技研究计划(No 20150628) （No 20150628）
河北医科大学研究生创新实验项目 （）
河北医科大学大学生创新实验项目资助的课题 （）
