中国真菌学杂志Issue(6)：336-339,4.
烟曲霉对人支气管上皮细胞作用及机制的初步研究
The evaluation of Aspergillus fumigatus infecting human bronchial epithelial cell in vitro
沈亮亮 1高清云 1黄欣 1郑建锋 1韩禹 1赵敬军1
作者信息
- 1. 同济大学附属同济医院皮肤科，上海 200065
- 折叠
摘要
Abstract
Objective To establish a model for the relative research that simulate Aspergillus fumigatus invading human bronchial epithelial cell (HBE)and analyze the change of HBE in different time.Methods Using Aspergillus fumigatus to infect HBE in vitro and researching the change rules (biological form of HBE by adding liquid supernatant from the model, the apoptosis rate of the cells by Fluorescence Activating Cell Sorter and apoptosis gene (Bax)expression by real-time PCR). Results As the action time of Aspergillus fumigatus infecting HBE gone by,the biological form of HBE has changed,the retractile and round cells grew in number,the apoptosis rate of the cells also increased gradually and BaK expression obvious-ly increased.Conclusion The changes of biological form,apoptosis rate and the Bax expression of HBE are evaluation system of simulating Aspergillus invading HBE in IPA which establishes in vitro and can use for relative basic and clinical research.关键词
支气管上皮细胞/烟曲霉/凋亡/凋亡基因Key words
human bronchial epithelial cell/Aspergillus fumigatus/apoptosis/apoptosis gene分类
医药卫生引用本文复制引用
沈亮亮,高清云,黄欣,郑建锋,韩禹,赵敬军..烟曲霉对人支气管上皮细胞作用及机制的初步研究[J].中国真菌学杂志,2015,(6):336-339,4.
