中国真菌学杂志Issue(6)：345-351,7.
基于 Hog-MAPK 信号通路探讨白念珠菌氟康唑耐药机制的实验研究
A Study of fluconazole resistance mechanism of Candida albicans based on Hog-MAPK signal pathway
摘要
Abstract
Obejectives To investigate the role of Hog-MAPK signaling pathway in the fluconazole resistance mechanism of Candida albicans mechanism.Methods Using Real-Time PCR and Western blot to compare the expression of HOG1 gene and Phosphorylation p38 MAPK protein from Hog-MAPK signaling pathway in fluconazole sensitive strain and resistance strain,then dectect the fluconazole MIC of Candida albicans HOG1 mutant and its original parent strain/standard strain.Re-sults The difference of the expression of Hog-MAPK signaling pathway related gene and protein is found between Candida albicans fluconazole sensitive strain and resistant strain,the expression of sensitive strain is less than resistant strain to a cer-tain extent,and HOG1 mutant strain is more sensitive to fluconazole.Conclusion The fluconazole resistance mechanism of Candida albicans may be associated with some gene and protein from Hog-MAPK pathway.The decreased expression of these genes and proteins may cause change of resistance to fluconazole.关键词
白念珠菌/Hog-MAPK 信号通路/耐药性Key words
Candida albicans/Hog-MAPK signaling pathway/drug resistance分类
医药卫生引用本文复制引用
闻轶旸,黄欣,沈帅帅,赵敬军..基于 Hog-MAPK 信号通路探讨白念珠菌氟康唑耐药机制的实验研究[J].中国真菌学杂志,2015,(6):345-351,7.基金项目
上海市科委自然科学基金(13ZR1437900) （13ZR1437900）
