中华灾害救援医学Issue(1)：12-16,5.DOI:10.13919/j.issn.2095-6274.2016.01.004
大鼠活体内 Luciferase 标记骨髓间充质干细胞生物发光示踪监测
In vivo monitoring of luciferase labeled bone marrow mesenchymal stem cells by bioluminescent in rats
摘要
Abstract
Objective To monitor the colonization and dynamic distribution of allogeneic bone marrow mesenchymal stem cells (BMSC) in healthy rats by using the technique of bioluminescent imaging. Methods BMSC from Wistar rat were infected by lentiviral which carry stable expression genes of luciferase and green fluorescence protein (Luc-GFP-BMSC), then implanted into Wistar rats by tail vein injection. Dynamic distribution of Luc-GFP-BMSC in rats was continuously monitored by using in vivo imaging system. Results In vivo imaging results showed that biological signal was strongest at 1.5 hours after implantation of Luc-GFP-BMSC in rats, and mainly resided in the lung, 24 hours later, signal in the back gradually enhanced and became dominant, after 3 days, signal in the chest couldn’t be detected, after 14 days, biological signal in the whole body disappeared. Conclusions After implantation through the tail vein, allogeneic BMSC in healthy Wistar rats initially reside in the lungs, and then egress to the back, finally the biological signal gradually disappeared, which suggests that tail vein injection of BMSC may be an effective approach for lung disease.关键词
骨髓间充质干细胞/生物发光/活体成像/慢病毒Key words
bone marrow mesenchymal stem cell/bioluminescent imaging/in vivo imaging/lentiviral分类
生物科学引用本文复制引用
曹娟,吕琪,丁辉,于梦洋,刘晋阳,樊毫军..大鼠活体内 Luciferase 标记骨髓间充质干细胞生物发光示踪监测[J].中华灾害救援医学,2016,(1):12-16,5.基金项目
天津市科技计划项目 （）
