广东海洋大学学报Issue(6)：1-8,8.DOI:10.3969/j.issn.1673-9159.2015.06.001
稀杯盔形珊瑚转录组分析
Transcriptome Analysis ofGalaxea astreata
摘要
Abstract
High-throughput sequencing technology Illumina HiSeqTM2000 was used to sequence the Galaxea astreata transcriptome,and 50 360 620 reads were got in all.And then assembled software Trinity was used to denovo assembled the reads,getting 81014 Unigenes and 60 471 Coding Sequences(CDs). Blasted with the four database Nr(Non-redundant), COG(Cluster of Orthologous Groups of proteins),KEGG(Kyoto encyclopedia of genes and genomes), Swissprot,36 545 annotated Unigenes were gotten. Blasted with COG database,14 491 unig- enes were gained and according to the function it was divided into 24 categories. 4 642 Unigenes join in the general function prediction only category, which own the most numbers of Unigenes. Blasted with KEGG pathway,16 021 Unigenes were annotated, and it was divided into 241 categories according to different pathways,including metabolic pathway, calcium signaling pathway, and MAPK signaling pathway and so on. Of all the pathways, the number of genes join in the metabolic pathways was maximum in 2450 (15.29%). GO(Gene Ontology)functional classification divided all Unigenes into 47 categories. Through this study,a lot of Galaxea astreata coral Unigenes were gotten. It can help us find some new functional genes and to know about the growth of coraland adaptation to the environment changes.关键词
转录组/稀杯盔形珊瑚/Unigene/信号通路Key words
transcriptome/Galaxea astreata/Unigene/signal pathway分类
农业科技引用本文复制引用
刘金豆,刘丽,黄元佳..稀杯盔形珊瑚转录组分析[J].广东海洋大学学报,2015,(6):1-8,8.基金项目
国家海洋公益性行业科研专项(201105012) （201105012）
广东省自然科学基金项目(S2011010000269) （S2011010000269）
广东省海洋渔业科技推广专项(A201308E02) （A201308E02）
