肿瘤药学Issue(6)：430-435,6.DOI:10.3969/j.issn.2095-1264.2015.06.07
牛蒡子苷元通过抑制PI3-K/Akt信号通路诱导肝癌细胞凋亡
Arctigenin, a Natural Lignan Compound, Induces Apoptotic Death of Hepatocellular Carcinoma Cells via Suppression of PI3-K/Akt Signaling
摘要
Abstract
Objective To explore the effects of arctigenin, a natural lignan compound, on the growth of human hepatocellular carcinoma (HCC) cells and the possible mechanisms. Methods The HepG2 and Hep3B cells were treated with different concentrations of arctigenin. The MTT assay was applied to detect the cell proliferation of HCC cells. Flow cytometry was applied to detect the cell apoptosis and cell cy-cle, and Western blot was adopted to detect the expressions of caspase-3 and caspase-9 as well as the expressions of anti-apoptotic proteins in cells. Manipulating Akt signaling was used to determine its role in the action of arctigenin. Results Arctigenin significantly inhibited the proliferation and induce the apoptosis of HCC cells in a concentration-dependent manner. Arctigenin induced the activation of caspase-9 and caspase-3. Overexpression of a constitutively active Akt mutant blocked arctigenin-induced apoptosis. Combinational treatment with arctigenin and the PI3K inhibitor LY294002 enhanced apoptosis significantly. Arctigenin reduced the expression of Bcl-xL, Mcl-1 and sur-viving, and the phosphorylation of mTOR and S6K, which were significantly reversed by overexpression of constitutively-active Akt. Con-clusion Arctigenin could down-regulate the expressions of anti-apoptotic proteins and promote the apoptosis of hepatocellular carcinoma cells by inactivating PI3-K/Akt signaling.关键词
牛蒡子苷元/原发性肝癌/细胞凋亡Key words
Arctigenin/Hepatocellular carcinoma/Apoptosis分类
医药卫生引用本文复制引用
王静泓,姜孝新,曾乐平,刘炼,周辉,刘玉冰..牛蒡子苷元通过抑制PI3-K/Akt信号通路诱导肝癌细胞凋亡[J].肿瘤药学,2015,(6):430-435,6.基金项目
湖南省自然基金资助项目（14JJ2092）。 （）
