广东医学2015，Vol.36Issue(24)：3737-3741,5.
IκBα低表达转基因小鼠的建立及其表达调控
Establishment of transgenic mice with IκBα low expression and the regulatory mechanism of the IκBα expression
摘要
Abstract
Objective In order to study the whole biological function of IκBαin vivo, we developed an IκBα low expression transgenic mice and analyze the regulatory mechanism of IκBα expression .Methods The targeting vector RV-IκBα-Loxp-Mut-Neo was constructed , then the linearized vector was injected into embryonic stem cells .South-ern blotting was used to screen the positive recombinant clones .Then the positive clone was microinjected into blastocysts to generate chimeric mice.Cross-breeding the chimeric with wild -type (C57BL/6N) mice generated heterozygous IκBα knockin mice .Homozygous IκBα knockin mice were cross -bred with Ella Cre mice to generate offspring , which interbred to generate homozygotes whose interval between two Loxp sites was deleted .The genetic type was identified with PCR . Western blot was used to detect expression of IκBα in various organs of transgenic mice .Results The IκBα knockin and IκBαknockout transgenic mice were established by PCR detection .The Western blot showed that the expression of IκBα protein in the spleen, thymus and lung of the homozygous IκBα knockin mice was lower than that in wild -type mice.A-part from the above organs , the mutant chromatid expression of IκBα protein in the skin , nasopharynx and stomach of the heterozygous IκBα knockin mice was also lower .Expression of IκBα protein was lower in most organs of IκBα knockout mice.Conclusion The transgenic mouse with low expression of IκBα was successfully established in the present study , and preliminary analysis of regulation of IκBα expression was implemented .关键词
IκBα/低表达/转基因/调控机制Key words
IκBα gene/low expression/transgenic mice/regulatory mechanism引用本文复制引用
刘青言,夏机良,肖东,彭白露..IκBα低表达转基因小鼠的建立及其表达调控[J].广东医学,2015,36(24):3737-3741,5.基金项目
国家自然科学基金资助项目(编号:31070773) （编号:31070773）
