广东医学2015，Vol.36Issue(24)：3750-3753,4.
丹酚酸B对阿霉素诱导H9c2细胞毒性的保护作用及其作用靶点
The protective effects of salvianolic acid B against doxorubicin-induced cardiotoxicity in H9c2 cells and the pre-diction of its targets
摘要
Abstract
Objective To investigate the protective effects of salvianolic acid B ( Sal B ) against doxorubicin (Dox)-induced cardiac injury in H9c2 cells and its possible targets.Methods H9c2 cells treated with Dox and Sal B were used to evaluate cell protective effects of Sal B .MTT assay and PharmMapper sever were used to observe the cell via-bility and to screen the potential targets of Sal B , respectively .The predicted results were further evaluated by molecular docking program Autodock .Results Sal B at the concentrations of 100 μmol/L and 300 μmol/L significantly protected H9c2 cells from Dox-induced cell toxicity .The data from PharmMapper server and molecular docking analysis demon-strated that mitogen-activated protein kinase 12 ( MAPK12 ) and DNA topoisomerase 2 might be the potential targets of Sal B on the protective effect against Dox .Conclusion Sal B prevents H9c2 cells from Dox-induced H9c2 cell toxicity, and MAPK12 and DNA topoisomerase 2 may be involved in this action .关键词
丹酚酸B/心脏保护/分子对接/丝裂原活化蛋白激酶磷酸酶12/DNA拓扑异构酶2Key words
salvianolic acid B/cardioprotection/docking/mitogen -activated protein kinase 12/DNA topoi-somerase 2引用本文复制引用
卓贤进,申慧芳,方志潮,丁云岗,王滔,崔国祯..丹酚酸B对阿霉素诱导H9c2细胞毒性的保护作用及其作用靶点[J].广东医学,2015,36(24):3750-3753,4.基金项目
国家自然科学基金资助项目(编号:81460552),广东省潮州市卫生局科研基金资助项目(编号:201335) （编号:81460552）
