湖南中医药大学学报2015，Vol.35Issue(12)：18-22,5.DOI:10.3969/j.issn.1674-070X.2015.12.005
黄芪多糖和三七总皂苷配伍对糖尿病大鼠肾组织Ⅳ型胶原及层黏连蛋白表达的影响
Effect of Astragalus Polysaccharides Combined with Panax Notoginseng Saponins on the Expression of Col-Ⅳ and LN in Renal Tissues of Diabetic Rats
摘要
Abstract
Objective To investigate the effect of astragalus polysaccharides (APS) combined with panax notoginseng saponins (PNS) on the expression of Col-IV and LN in renal tissues of diabetic rats. Methods Diabetic rat model was induced by injecting streptozotocin intraperitoneally. The diabetic rats were randomly divided into the model, APS, PNS, APS+ PNS, benazepril groups. The rats were administered medicine intragastrically one time a day for 8 weeks. Urinary protein level in 24h, blood sugar, blood creatinine and urea nitrogen were determined. The protein expression of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor (PDGF-BB), type IV collagen (Col-IV) and laminin (LN) in the renal tissue were determined by immunohistochemistry. Results Compared with the normal control group, unrinary protein level in 24 h, blood glucose, creatinine and urea nitrogen level in rats in the model group were significantly higher than those in normal control group (P<0.01); The protein expression of TGF-β1, PDGF-BB, Col-IV and LN in the renal tissue were enhanced (P<0.01). Compared with the model group, unrinary protein level in 24 h, blood glucose, creatinine and urea nitrogen level were reduced (P<0.01); The protein expression of TGF-β1, PDGF-BB, Col-IV and LN in the renal tissue in rats were decreased significantly (P<0.01); The effects of APS combined with PNS were better than those of APS and PNS alone groups (P<0.05, P<0.01). Conclusion The combination between APS and PNS had protective effect on kidney of diabetic rats. Its mechanism may be related to inhibiting extracellular matrix synthesis by downregulating the protein expression of TGF-β1, PDGF-BB, Col-IV and LN.关键词
黄芪多糖/三七总皂苷/配伍研究/糖尿病大鼠/转化生长因子β1/血小板源性生长因子/Ⅳ型胶原蛋白/层粘连蛋白Key words
astragalus polysaccharides/panax notoginseng saponins/compatibility study/diabetic rats/transforming growth factor beta 1/platelet-derived growth factor/collagen type Ⅳ/laminin分类
医药卫生引用本文复制引用
蒋赛,彭晓珊,黄志华,王硕辉,谢月,唐映红..黄芪多糖和三七总皂苷配伍对糖尿病大鼠肾组织Ⅳ型胶原及层黏连蛋白表达的影响[J].湖南中医药大学学报,2015,35(12):18-22,5.基金项目
湖南省大学生研究性学习和创新性实验计划项目(湘教通[2012]402-187) （湘教通[2012]402-187）
2012地方高校国家级大学生创新训练计划项目(201210541005). （201210541005）
