湖南中医药大学学报2015，Vol.35Issue(12)：53-55,3.DOI:10.3969/j.issn.1674-070X.2015.12.015
不同炮制方法对黄精多糖含量的影响
Effcet of Different Processing Methods on the Content of Polygahatous Polysaccharides
摘要
Abstract
Objective To explore different processing methods on polysaccharide content of Polygonatum sibiricum Red. and Polygonatum cyrtonema Hua, which cultivated and processed in Hunan. Methods The polysaccharide was extracted by ultrasonic extraction and the protein was removed by Sevage's method. The content of polysaccharide was determined by phenol-sulfuric acid method. Results The polysaccharide content of Polygonatum sibiricum Red. with different times of steaming and drying processing methods were 7.973%, 3.584%, 2.845%, 2.194%, and the polysaccharide content of Polygonatum cyrtonema Hua with different processing methods were 9.602%, 3.185%, 2.572%, 2.043%; The precision was good (RSD=0.274%); The average recovery rate was 99.29%; the test has good stability in 60min (RSD=1.36%). Conclusion The content of polysaccharide was reduced with the more times of steaming and drying processing methods.关键词
黄精/多花黄精/黄精多糖/炮制/苯酚-硫酸法/酶标仪Key words
Polygonatum sibiricum Red./Polygonatum cyrtonema Hua/polysaccharide/phenol-sulfuricacid method/microplate spectrophotometer分类
医药卫生引用本文复制引用
瞿昊宇,冯楚雄,谢梦洲,朱建平,孙毅中,崔培梧,肖作为..不同炮制方法对黄精多糖含量的影响[J].湖南中医药大学学报,2015,35(12):53-55,3.基金项目
湖南省教育厅创新平台基金项目(12k083) （12k083）
湖南中医药大学中医诊断学国家重点学科开放基金项目(2014-27). （2014-27）
