林产化学与工业2015，Vol.35Issue(6)：108-112,5.DOI:10.3969/j.issn.0253-2417.2015.06.018
利用基因组DNA 诱变技术选育高抑制物耐受性工业酵母菌
Breeding of Robust Inhibitor-tolerant Industrial Saccharomyces cerevisiae Strain by Genomic DNA Mutagenesis
摘要
Abstract
The recombinant Saccharomyces cerevisiae HN-1-24 was obtained by combination of chemical mutagenesis and genomic DNA mutagenesis-based genetic recombination methods to improve the inhibitor tolerance and fermentation performance. The recombinant strain HN-1-24 exhibited a high conversation rate of sugar to ethanol and ethanol production under a condition with 7 g/L acetic acid and 1 g/L furfural. Thus 38 g/L of ethanol could be obtained from 100 g/L of glucose. When ethanol fermentation of cellulose hydrolyzate culture was performed,the fermentation time of the recombinant strain was decreased by 6 h and the final ethanol concentration was enhanced by 8. 8% as compared with the original strain. The results of this study provided a basis for the large-scale production of fuel ethanol by using lignocellulose.关键词
酿酒酵母/乙醇/化学诱变/遗传重组/抑制物Key words
Saccharomyces cerevisiae/ethanol/chemical mutagenesis/genetic recombination/inhibitor分类
化学化工引用本文复制引用
陈亮,徐龙龙,闫德冉,杜凤光,惠丰立..利用基因组DNA 诱变技术选育高抑制物耐受性工业酵母菌[J].林产化学与工业,2015,35(6):108-112,5.基金项目
国家自然科学基金资助项目(31370073) （31370073）
车用生物燃料技术国家重点实验室开放课题(2013021) （2013021）
