中国药房2016，Vol.27Issue(1)：76-79,4.DOI:10.6039/j.issn.1001-0408.2016.01.25
多指标优选益气固本颗粒澄清工艺
Optimization of the Purification Process of Yiqi Guben Granules with Multiple Indexes
摘要
Abstract
OBJECTIVE:To optimize the purification process of the extract from Yiqi guben granules. METHODS:The purifi-cation effect of the process was investigated with transfer rate of polysaccharide,calycosin glucoside and dry paste as evaluation in-dexes,using ZTC1+1 type Ⅱ,and shell poly sugar and 95% ethanol as clarifying agents. The purification process of the extract from Yiqi guben granules was optimized by orthogonal test using the ratio of material to liquid,the amount of clarifying agent and standing time as factor. The validation test was conducted. RESULTS:Selecting ZTC1+1 type Ⅱ as a clarifying agent,the best trans-fer rate of effective component had been obtained;optimal purification process was as follows as the ratio of material to liquid 1:2, the ratio of ZTC1+1 type Ⅱ A liquid 5%,the ratio of B liquid 10%,standing time of 5 h. The results of verification test showed transfer rates of dry paste in 3 tests were 71.54%,70.98%,69.21%,respectively;those of polysaccharide were 82.55%, 81.78%,82.15%,respectively;those of calycosin glucoside were 91.92%,92.34%,91.58%,respectively (all RSD≤1.72%, n=3). CONCLUSIONS:The optimized purification process is effective,stable and practical,and can be used for the purification of the extract from Yiqi guben granules.关键词
益气固本颗粒/ZTC1+1Ⅱ型澄清剂/澄清工艺/正交试验Key words
Yiqi guben granules/ZTC1+1 typeⅡclarifying agent/Purification process/Orthogonal test分类
医药卫生引用本文复制引用
沈涛,李季文,梁海宁,张小华,毕映燕..多指标优选益气固本颗粒澄清工艺[J].中国药房,2016,27(1):76-79,4.基金项目
甘肃省科技支撑计划项目(No.1304FKCA105) （No.1304FKCA105）
