摘要
Abstract
Objective To investigate the mechanism of ginsenoside Rb1 on the proliferation and apoptosis of human leukemia KG1a cell. Methods KG1a cells in the logarithmic growth phase were taken as the blank control group. The Rb1 group of the ginsenoside monomer was added to 20,40,80,160 Rb1 μmol/L. CCK-8 method was used to detect the proliferation of KG1a cells;apoptosis was detected by flow cytometry;Western Blot was used to detect the express of HDAC3,Bcl-2 and Caspase-3. Results The inhibition ef-fect of Rb1 on the proliferation of KG1a cells was significantly inhibited in vitro;in the range of 20-160 μmol/L Rb1,the inhibition effect was concentration dependent,and the inhibition was most obvious at 48 h;flow cytometry showed that with the increase of drug concentration,ROS level increased significantly ( P < 0. 05),and the apoptosis rate of KG1a cells was significantly increased,and the percentage of apoptotic cells and apoptotic cells was significantly different from that of the 0 μg/mL group( P < 0. 05);Western Blot showed that the expression of HDAC3 decreased significantly with the increase of drug concentration( P < 0. 05),while the expression of Caspase-3 and Bcl-2 increased. Conclusion Ginsenoside Rb1 can inhibit the proliferation of KG1a cells,and the inhibition may be achieved by promoting the apoptosis of KG1a cells.关键词
人参皂苷Rb1/去乙酰化酶3/凋亡Key words
ginsenoside Rb1/HDAC3/apoptosis分类
医药卫生
