重庆医学Issue(2)：159-163,5.DOI:10.3969/j.issn.1671-8348.2016.02.005
自体内皮祖细胞促进组织工程骨血管化的体内外实验研究
Autologous endothelial progenitor cells promote the neovascularization of tissue engineering bone in vitro and vivo
摘要
Abstract
Objective Investigate the ability of autologous endothelial progenitor cells (EPCs) in promoting the neovascular-ization of tissue engineering bone in vitro and vivo .Methods Co-culture EPCs drived from autologous peripheral blood and mesen-chymal stem cells drived from bone marrow (BMSCs) at the best proportion of 1 ∶ 2 which was the largest cell proliferation rate in vitro ,osteogenesis related cytokines Osteonectin ,Osteopotin ,Collagen Type 1(Col-1) and angiogenesis related cytokine VEGF in vitro by using real-time quantitative polymerase chain reaction(PCR) ,and compared with pure EPCs and BMSCs groups at 3rd ,7th and 14th day ;the tissue engineering bone seeded with EPCs ,BMSCs and co-culture cells (EPCs ∶ BMSCs = 1 ∶ 2) were transplanted into rabbit limbs muscle ,the growing states of tissue engineering bone were observed at 2 ,4 and 8 weeks after transplantation ,at the same time the expression of CD34 ,CD105 and ZO-1 were detected with immunohistochemistry staining .Results The mRNA expression of Osteonectin ,Osteopotin ,Col-1 and VEGF were gradually increased when detected at 3rd ,7th and 14th day with real-time PCR ,and the co-culture cells group increased most obviously in the three groups in vitro at the same period time(P< 0 .01) ;The microvascularization of the engineering biological bone were observed in vivo with immunohistochemistry ,and neovasculariza-tion of co-culture cells group was also the most obvious group in three groups ,immunohistochemical showed that CD34 ,CD105 and ZO-1 was also higher than the other two groups(P< 0 .01) .Conclusion Autologous EPCs interact with BMSCs could promote vas-cularization of tissue engineering bone both in vivo and in vitro .关键词
干细胞/内皮 ,血管/间充质干细胞/新生血管化 ,生理性/组织工程骨Key words
stem cells/endothelium ,vascular/bone marrow mesenchymal stem cells/neovascularization ,physiologic/tissue engineered bone分类
医药卫生引用本文复制引用
吴莉,赵娴,柯腾飞,陈泽谷,陆林,魏韩笑,张承磊,刘流..自体内皮祖细胞促进组织工程骨血管化的体内外实验研究[J].重庆医学,2016,(2):159-163,5.基金项目
国家自然科学基金资助项目（81460298）；云南省科技厅-昆明医科大学应用基础研究联合专项（2015 FB 038）。 （）
