汪海洋 1陈宇诗 1金大春 1张金栋 1刘颖菊 2杜永洪1
作者信息
- 1. 重庆医科大学生物医学工程学院/省部共建国家重点实验室培育基地 - 重庆市超声医学工程重点实验室/重庆市生物医学工程学重点实验室 400016
- 2. 重庆医科大学药学院 400016
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摘要
Abstract
Objective To investigate whether luteolin ease anti-inflammatory and anti-fibrosis of unilateral ureter obstruction of kidney in rats and its therapeutic effect on obstructive nephropathy .Methods Thirty two SD rats were randomly assigned into four groups as follows :sham operation group(n= 8) ,model group(n= 8) ,dexamethasone group(n= 8) and luteolin group(n= 8) . The sham group were exposed to left ureter separation operation ,while the other three groups were treated with left ureter ligation operation .Then four groups received drug as the operation demanded .Rats were sacrificed at the 10th day after we had got the blood and collected obstructed lateral kidney .Then colorimetric determination was taken to test the changes of serum nitrogen and serum creatinine ,and the kidney tissue were stained with HE and Masson to assess histology pathological changes .We also assess the severity of renal interstitium by using the index of intestinal damage and fibrosis .Results Compared with sham group ,we could observe several changes in model group as follows :the obvious enlargement of the kidney ,cortex thinning ,the deformation of renal tubular ,and the evident inflammatory reaction ,as well as the significant increase of the index of intestinal damage and fibrosis inte-gral of renal interstitium increased(P< 0 .05) ,serum nitrogen and creatinine (P< 0 .05) .Similar to model group ,such changes were observed in the lueteolin group and dexamathasone group :rats′ kidney were enlarged ,inflammatory reaction ,the index of intestinal damage and fibrosis integral of renal interstitium decreased significantly(P< 0 .05) ,the luteolin group serum nitrogen and creatinine increased significantly (P< 0 .05) ,yet that in the dexametasone group was not significant ;contrasted with model group ,whereas , luteolin group and dexamethasone group kidneys′ ,volume diminished distinctly ,but much bigger than those in sham group ,cortex thinning was less obvious ,the inflammatory reaction weakened distinctly ,as well as the renal tubular decoration and the index of in-testinal damage .Fibrosis integral of renal interstitium decreased significantly ( P < 0 .05) ,the serum nitrogen and creatinine de-creased significantly (P < 0 .05) ,there was remarkable difference between the dexamethasone group and the luteolin group(P <0 .05) .Conclusion Luteolin can′t completely prevent or reverse unilateral ureter obstruction rat kidney tissue damaging and func-tional changing ,but it can ease the unilateral ureter obstruction rats inflammatory reaction ,kidney destruction as well as renal path-ological damaging .Its therapeutic effect is inferior than that of dexamethasone .关键词
木犀草素/地塞米松/输尿管梗阻/炎症/纤维化Key words
luteolin/dexamethasone/ureteral obstruction/inflammation reaction/fibrosis分类
医药卫生
