重庆医学Issue(3)：293-295,298,4.DOI:10.3969/j.issn.1671-8348.2016.03.002
骨形态发生蛋白在胶原诱导关节炎小鼠模型中的表达
Expression of bone morphology proteins in mice with collagen-induced arthritis
摘要
Abstract
Objective To establish collagen‐induced arthritis (CIA) model used of DBA/1J mouse ,a preliminary study of the expression levels of BM Ps in mononuclear cells of peripheral blood and the joints tissue of CIA mice .Methods We induced DBA/1J mice and developed arthritis pathology by using of bovine type Ⅱ ;then ,we detected mRNA and protein expression levels of BMPs by using quantitative PCR and immunohistochemical staining .Results We successfully established CIA mouse model .The expres‐sion of BMP4 and BMP9 mRNA was significantly down‐regulated in peripheral blood mononuclear cells and in the pathogenesis of joint tissues of CIA mice (P<0 .05) .It showed that BMP9 protein significantly decreased in joint tissues of CIA mice by immuno‐fluorescence technique (P=0 .002) .Conclusion At the genetic level ,the expression of BMP4 and BMP9 could be significantly down‐regulated in the CIA mouse .At the protein level ,BMP9 could be significantly down‐regulated in the CIA mouse .关键词
骨形态发生蛋白/聚合酶链反应/CIA小鼠Key words
bone morphogenetic proteins/polymerase chain reaction/CIA mice分类
医药卫生引用本文复制引用
刘强,张莹莹,焦志军,顾金金..骨形态发生蛋白在胶原诱导关节炎小鼠模型中的表达[J].重庆医学,2016,(3):293-295,298,4.基金项目
国家自然科学基金（81373217）；江苏大学2012年度医学临床科技发展基金（JLY20120169）。 （）
