重庆医学Issue(3)：299-302,4.DOI:10.3969/j.issn.1671-8348.2016.03.004
损伤性肾匀浆体外诱导大鼠骨髓间充质干细胞转分化效果及可能机制的研究
Transdifferentiation of rat bone marrow mesenchymal stem cell induced by injured kidney homogenate in vitro and the mechanism study
摘要
Abstract
Objective To explore the effect of transdifferentiation of bone marrow‐derived mesenchymal stem cells(BMSCs) and the possible mechanism ,the micro‐environment of acute kidney injury in vitro was simulated with injured kidney homogenate . Methods BMSCs ,isolated from rat bone marrow ,were cultured with the presence of 10% injured kidney homogenates .After seven days′culturing ,the morphological changes were observed under microscope .The expression of CK‐18 ,hepatocyte growth factor (HGF) and bone morphogenetic proteins‐7(BMP‐7) were tested by qPCR and Western blot respectively .Results The morphologi‐cal observation showed that the BMSCs of induced group had been changed obviously after 7 days incubation .The expression of CK‐18 induced group were significantly upregulated .The protein levels of HGF and BMP‐7 both in cell culture medium and in induced cell were increased sharply .Conclusion BMSCs could be transdifferentiated partly into renal tubular‐like epithelial cells after incu‐bation .It figures out the possible mechanism that BMSCs may produce “kidney protection factors” such as HGF and BMP‐7 by their paracrine .关键词
间质干细胞/上皮细胞/肾小管/骨髓间充质干细胞/急性肾损伤/转分化Key words
mesenchymal stem cells/epithelial cells/kidney tubules/bone marrow mesenchymal stem cells/acute kidney inju-ry/transdifferentiation分类
医药卫生引用本文复制引用
陈昌金,杨雪梅,吕春燕,赵梓亦,袁惠,辜海英,高泓,陈奕名,陈明..损伤性肾匀浆体外诱导大鼠骨髓间充质干细胞转分化效果及可能机制的研究[J].重庆医学,2016,(3):299-302,4.基金项目
成都市科技局项目（313-106）；成都中医药大学附属医院项目（Y2009003）。 （）
