重庆医学Issue(3)：317-319,3.DOI:10.3969/j.issn.1671-8348.2016.03.009
美托洛尔对小鼠心肌缺血再灌注损伤的保护机制研究
Protective effect of metoprolol on ischemia-reperfusion induced myocardial tissue injury in mice
摘要
Abstract
Objective To investigate the possible mechanism of metoprolol on protecting against ischemia‐reperfusion in‐duced injury .Methods A total of 32 healthy 3-4 months male C57BL/6 mice were divided into four groups(n=8)as following :Sham‐operating group(control group);metoprolol group;ischemia‐reperfusion group(I/R group);metoprolol + I/R group .Myo‐cardial injury ,apoptosis ,cytochrome c release ,Caspase‐3 activity and calpain activity were determined in these groups .Results Al‐though there was no obvious changes in the regions at risk between I/R group and metoprolol + I/R group ,metoprolol pretreat‐ment significantly reduced the ratio of the infarct to risk regions(P<0 .05) .In the I/R group ,the rate of cardiomyocyte apoptosis , cytochrome c release ,as well as the activity of Caspase‐3 and calpain significantly increased compared to the control group(P<0 .01) .However ,these effects of I/R injury were alleviated by pretreatment with metoprolol .Conclusion metoprolol might protect against ischemia‐reperfusion induced injury by preventing calpain activation .关键词
心肌/心肌缺血/心肌再灌注/美托洛尔/钙蛋白酶/细胞凋亡Key words
myocardium/myocardial ischemia/myocardial reperfusion/metoprolol/calpain/apoptosis分类
医药卫生引用本文复制引用
李强,岳荣川,罗涛,陈海燕,胡厚祥,徐继前,谭春燕,徐磊,张双,王欢,张荣驿..美托洛尔对小鼠心肌缺血再灌注损伤的保护机制研究[J].重庆医学,2016,(3):317-319,3.基金项目
国家自然科学基金项目（81070101）；四川省教育厅课题（12ZA060）。 （）
