重庆医学Issue(3)：323-325,3.DOI:10.3969/j.issn.1671-8348.2016.03.011
雌二醇通过BDNF促进端脑神经细胞增殖与分化
Estradiol induce neural proliferation and differetiation through regulating the expression of BDNF
摘要
Abstract
Objective To address the influences of estradiol on the proliferation and neuronal differentiation in neural tis‐sues ,and then analyse whether BDNF involves in the pathway of this estradiol‐induced cell division and specification .Methods The forebrain tissues of experimental animals were cultured in vitro ,and treated respectively with estradiol ,estrogen receptor inhibitor , BDNF ,BDNF antibody ,or received a combined treatment .And the immunohistochemistry was performed to reveal the situation of cell proliferation ,neuronal differentiation in different experimental groups .Then real‐time PCR was carried out to detect whether the expression of BDNF was regulated by estradiol .Results Both estradiol and BDNF increased the newborn cells and Hu positive neurons in cultured tissues ,estrogen receptor inhibitor tamoxifen and BDNF antibody decreased the measurements conversely ;the effects of estradiol were diminished by anti‐BDNF ,while BDNF′s effect didn′t changed by tamoxifen .Moreover ,the expression of BDNF mRNA were up‐regulated by estradiol and were inhibited by estrogen receptor inhibitor .Conclusion Estradiol might induce neural proliferation and differentiation in vitro by regulating the level of BDNF .关键词
雌二醇/脑源性神经营养因子/神经组织Key words
estradiol/brain-derived neurotrophic factor/neural tissue分类
医药卫生引用本文复制引用
陈琼,林丽英,唐晓华,郭旭光,夏勇..雌二醇通过BDNF促进端脑神经细胞增殖与分化[J].重庆医学,2016,(3):323-325,3.基金项目
广州市医药卫生科技项目（20131A011160）；广州医学院第三附属医院博士启动项目（2012Y19）。 （）
