重庆医学Issue(3)：342-344,347,4.DOI:10.3969/j.issn.1671-8348.2016.03.017
龙葵碱联合VEGF抗体对人结肠癌鸡胚移植模型血管生成的影响
Effect of angiogenesis on Solanine and VEGF antibody in chicken embryo transplantation model of human colon cancer cells
摘要
Abstract
Objective To establish model of the chicken embryo transplantation of human colon cancer cells ,and investigate the effect of Solanine、VEGF antibody and Solanine combined with VEGF antibody on human colon cancer cells induce tumor angio‐genesis and tumor proliferation .Methods The model of the chicken embryo transplantation of human colon cancer HT‐29 cells were divided into three experimental group and control group .We added to the chick embryo chorioallantoic membrane with Sola‐nine、VEGF antibody and Solanine+ VEGF antibody mixture ,PBS was added to the control group .Then we analysed picture through the stereomicroscope and IPP 6 .0 image analysis software ,using immunohistochemistry envision method to detect of CD34 antigen and ki‐67 antigen ,and observing effect of Solanine group ,VEGF antibody group ,Solanine+ VEGF antibody group and the effect on the tumor angiogenesis and tumor proliferation .Results The tumor angiogenesis ,CD34 antigen and ki‐67 antigen of Sola‐nine+VEGF antibody group were significantly better than those of VEGF antibody group and Solanine group(P<0 .01);VEGF antibody group had statistical significant difference with Solanine group(P<0 .01);the effect of other three groups were better than that of the control group(P<0 .01) .Conclusion Solanine、VEGF antibody and Solanine combined with VEGF antibody could in‐hibit tumor angiogenesis and tumor proliferation of human colon cancer cell line HT‐29 to induce .It provides a new way for anti‐an‐giogenes .关键词
龙葵碱/人结肠癌 HT-29细胞/VEGF抗体/肿瘤血管生成/CAMKey words
solanine/HT-29 human colon cancer cells/VEGF antibody/tumor angiogenesis/chick embryo chorioallantoic membrane分类
医药卫生引用本文复制引用
杨雪峰,邓冬雪,张桃,宁伟伟,郑兴斌,谢铭..龙葵碱联合VEGF抗体对人结肠癌鸡胚移植模型血管生成的影响[J].重庆医学,2016,(3):342-344,347,4.基金项目
贵州省卫生厅科学技术基金（gzwkj2012-1-112）；遵义医学院中青年基金（院字［2009］号）。 （）
