重庆医学Issue(3)：351-353,3.DOI:10.3969/j.issn.1671-8348.2016.03.020
流式细胞术分析骨髓增生异常综合征小巨核细胞的初步研究
Study on micromegakaryocytes of patients with myelodysplastic syndrome by flow cytometry
摘要
Abstract
Objective To setup a measurement of human bone marrow micromegakaryocyte which based on CD41a and PI double‐labeled flow cytometric analysis ,and study the significance in the diagnosis of MDS .Methods In 42 cases of MDS patients , their bone marrow megakaryocytes were obtained by Percoll density gradient separation medium .The megakaryocyte glycoproteinⅡb/Ⅲa(CD41a)were marked with fluorescein isothiocyanate through its corresponding monoclonal antibody ,and their DNA were marked with PI .Then the megakaryocyte ploidy was analyzed by flow cytometry(FCM ) .Results The method for micromegakaryo‐cyte identification and analysis was established .In 42 patients with MDS ,the detection rate of micromegakaryocyte was 90 .5 per‐cent by FCM analysis ,but only 54 .8 percent by Wright‐Giemsa staining test and 64 .3 percent by immunohistochemistry ,the differ‐ence among them was statistically significant(χ2 = 13 .640 ,P= 0 .001) .The 42 patients with MDS were divided into two groups (low‐risk group and high‐risk group) .The detection rates of micromegakaryocyte were 81 .8 percent in low‐risk group and 100 per‐cent in high‐risk group separately by FCM analysis ,the difference was statistically significant(χ2 =4 .019 ,P=0 .045) .Conclusion The detection rate of micromegakaryocyte by FCM with CD41a and PI double marker is higher than that by cytochemical staining . The detection rate of micromegakaryocyte in the high‐risk group is higher than that of the low‐risk group ,which shows that the de‐tection of micromegakaryocyte is of great significance for MDS prognosis assessment .关键词
流式细胞术/骨髓增生异常综合征/巨核细胞/DNA倍体/CD41aKey words
flow cytometry/myelodyplastic sydrome/megakaryocyte/DNA ploidy/CD41a分类
医药卫生引用本文复制引用
刘帅,曹征,张晓飐,岳保红..流式细胞术分析骨髓增生异常综合征小巨核细胞的初步研究[J].重庆医学,2016,(3):351-353,3.基金项目
国家自然科学基金资助项目（81271911）；河南省医学科技攻关项目（201002006）。 （）
