动物医学进展Issue(2)：11-14,4.
副猪嗜血杆菌 ompP2基因在猪肺泡巨噬细胞中的表达
Expression of Haemophilus parasuis ompP2 Gene in Porcine Alveolar Macrophages
摘要
Abstract
In order to study the function of Haemophilus parasuis (HPS)ompP2 gene,a pair of primers were designed and synthesized according to nucleotide sequence of HPS ompP2 gene.The ompP2 gene of HPS LZ was amplified strain.The amplified ompP2 gene was cloned into pEASY-Blunt vector.The ompP2 gene was sequenced and cloned into the eukaryotic expression vector pCI-neo.The recombinant plasmid was used to transform competent Trans-T1 and the positive clones were identified by restriction enzyme di-gestion.Thus,the recombinant pCI-neo-ompP2 was transfected to porcine alveolar macrophages (PAM). The expression of pCI-neo-ompP2 was detected by RT-PCR and Western blot.The result showed that a 1 158 bp ompP2 gene fragment was amplified from the HPS DNA.The eukaryotic expression vector pCI-neo-ompP2 containing ompP2 gene was constructed successfully.The 1 000-2 000 bp DNA was amplified from the mRNA extracted from the PAM transfected with pCI-neo-ompP2 by RT-PCR and the 42 ku pro-tein was detected by Western blot.This study provided an experiment fundation for studying the mecha-nism which HPS infects PAM.关键词
副猪嗜血杆菌/ompP2 基因/真核表达载体/猪肺泡巨噬细胞/表达Key words
Haemophilus parasuis/ompP2 gene/eukaryotic expression vector/porcine alveolar macro-phage/expression分类
农业科技引用本文复制引用
于江,王金宝,杜以军,李俊,丛晓燕,孙文博,时建立,陈智,陈蕾,吴家强..副猪嗜血杆菌 ompP2基因在猪肺泡巨噬细胞中的表达[J].动物医学进展,2016,(2):11-14,4.基金项目
山东省现代农业产业技术体系 （）
山东省自然科学基金(ZR2015YL078) （ZR2015YL078）
山东省农业科学院重大科技成果培育计划(2014CGPY04) （2014CGPY04）
山东省农业科学院青年科研基金项目(2015YQN51) （2015YQN51）
