动物医学进展Issue(2)：32-37,6.
2011年－2014年我国部分省区 H9N2亚型禽流感病毒HA 基因序列分析
Sequence Analysis of HA Genes of H9N2 Subtype Avian Influenza Virus from Parts of China During 201 1-2014
摘要
Abstract
In this study,49 H9N2 subtype avian influenza virus strains (AIVs)were collected from sick chickens in China during 201 1-2014.The HA genes of these virus were sequenced and the sequence were analyzed.The results showed that the open reading frame of all the HA genes contained 1 683 nucleotides coding for 560 amino acids.Phylogenetic analysis based on the HA genes showed that the 49 isolates be-longed to H9.4.2 lineage with the strain HK/Y280/97 as representative,and they were obviously clustered into two sub-lineages (H9.4.2.5 and H9.4.2.6 ).The HA gene homology of all the sequences were in 87.1%-100%.The nucleotide identity of the 49 virus strains with the vaccine strains of H9N2 AIV (SH/F/98,GD/SS/94,and SD/6/96)were between 89.4%-92.5%.Analysis of the cleavage site of the deduced HA amino acid sequences of the 49 isolates showed there were no polybasic residues,which met the char-acteristics of low-pathogenic avian influenza virus.The tested strains in this study had the receptor binding site motifs as PWTN∗LY.They had motifs NGLM/QGL on the left-edge of receptor-binding pocket,and GTSKA on the right-edge.The results showed that the strains carried potential glycosylation sites that were located at ten different amino acid positions,but only six of them were conservative.In the present study,our findings demonstrated the continued circulation of H9N2 AIVs in China and showed the H9.4. 2.5 sub-lineage viruses were prevalent strains since 2013.As variations of H9N2 genes were still found, continuing molecular epidemiological surveillance of H9N2 AIVs in China is necessary.关键词
H9N2 亚型禽流感病毒/HA 基因/序列分析Key words
Avian influenza virus H9N2 subtype/HA gene/sequence analysis分类
农业科技引用本文复制引用
陈顺艳,廖昌韬,曾凡桂,严专强,苏晓娜,廖秋生,覃健萍,陈峰..2011年－2014年我国部分省区 H9N2亚型禽流感病毒HA 基因序列分析[J].动物医学进展,2016,(2):32-37,6.基金项目
广东省自然科学基金团队项目( S2013030013313) （ S2013030013313）
