动物医学进展Issue(2)：64-69,6.
蛋白磷酸酶2A 的 Cβ亚基在胚胎成纤维细胞凋亡过程中的作用
Effects of Catalytic Subunit βof Protein Phosphatase 2A on Apoptosis of Embryonic Fibroblasts
摘要
Abstract
To investigate the effects of catalytic subunit βof protein phosphatase 2A (PP2A)on cell cycle and cell apoptosis in mouse embryo fibroblasts (MEFs),the mouse embryos at E12.5 by mating the heter-ozygotes of Cβ subunit conventional knockout male and female mice at the ratio of 1 ∶ 2 were obtained. MEFs were prepared by trypsin digestion and genotyped by PCR,RT-PCR and Western blot to identify the genetic basis of each embryo.FACs was carried out to determine the cell apoptosis and cell cycle, meanwhile,the anti-apoptosis protein Bcl-2 was checked by Western blot.Genotyping by PCR,RT-PCR and Western blot showed that both knockout and wildtype MEFs were isolated by trypsin digestion at the ratio of 3∶3.The MEFs at M1 stage was 46.94%±4.17 and 53.95%±2.81,respectively and M2 stage was 29.83%±3.25 and 21.14%±2.53.There is no significant difference in cell apoptosis between wildtype and Cβknockout MEFs and slight significant difference between wildtype and knockout MEFs in cell cycle.关键词
胚胎成纤维细胞/细胞凋亡/细胞周期Key words
mouse embryonic fibroblast/cell apoptosis/cell cycle分类
农业科技引用本文复制引用
王庆华,王生存,李斌,刘春,吴刘成,王旭,彭晓清,邵义祥..蛋白磷酸酶2A 的 Cβ亚基在胚胎成纤维细胞凋亡过程中的作用[J].动物医学进展,2016,(2):64-69,6.基金项目
江苏省高校自然科学研究面上项目(11KJB180010 （）
14KJB180018 （）
15KJB180015) （）
