动物医学进展Issue(2)：120-124,5.
宠物源大肠埃希菌的分离鉴定和耐药性研究
Isolation,Identification and Antimicrobial Resistance of Escherichia coli in Companion Animals
摘要
Abstract
The occurrence of antimicrobial-resistant Escherichia coli and related resistance characteristics were investigated in companion animals in Changchun.E .coli were isolated and identified from 135 anal swab samples from two pet hospitals.Triplex PCR was used to detect E .coli phylogenetic groups.The re-sistance to 1 9 antimicrobial agents and extended-spectrum β-lactamases(ESBLs)phenotype were detected by BD PhoenixTM-100 System.Among 95 isolates,78.9% showed resistance to ampicillin,76.8% showed resistance to piperacillin,followed by 61.6% showed resistance to tetracycline.More than 50% isolates showed resistance to cefazolin,cefotaxime,cefepime,gentamicin,trimethoprim-sulfamethoxazole,cipro-floxacin and levofloxacin.However,all these isolates were sensitive to imipenem and meropenem.Further-more,15 isolates were sensitive to all the antimicrobials tested (15.8%).63 isolates presented multidrug resistance phenotype (66.3%).53.7% of these isolates presented ESBL phenotype.This study provided the epidemiological characteristics of antimicrobial-resistant E .coli from pets in Changchun,which may be useful for companion animal treatment.关键词
大肠埃希菌/耐药性/超广谱β内酰胺酶/宠物Key words
Escherichia coli/antimicrobial resistance/extended-spectrumβ-lactamases/companion animal分类
农业科技引用本文复制引用
刘果,纪雪,孙洋,刘哲,祝令伟,刘军,周伟,郭学军,冯书章..宠物源大肠埃希菌的分离鉴定和耐药性研究[J].动物医学进展,2016,(2):120-124,5.基金项目
国家科技部传染病重大专项(2013ZX10004-217-002) （2013ZX10004-217-002）
863项目(2012AA022006) （2012AA022006）
吉林省科技计划项目(20150101110JC) （20150101110JC）
