动物医学进展Issue(2)：125-127,3.
貉源犬巴氏杆菌的分离与鉴定
Isolation and Identification of Pasteurella canis from Raccoon Dogs
摘要
Abstract
Four pathogenic strains were isolated from lungs,spleen,liver and kidney of the infected 7-10 days old aberdeen raccoon by routine bacterial isolation,identification technologies.The pathogen was i-dentified as Pasteurella multocida based on its morphology,culture characteristics,biochemical charac-teristics and 1 6S rRNA gene PCR results.Sequence alignments indicated that 1 6 S rRNA gene from the i-solates shared 97.8%-100% identity with Pasteurella canis in GenBank and located in a relatively inde-pendent branch.Artificial infection experiment confirmed that the isolates were pathogenic.The result of drugs sensitivity test showed that the isolates were highly sensetive to penicillin,cephalosporins and fluo-roquinolones,resistant to streptomycin,tetracycline,oxytetracycline and sulfadiazine sodium.This repre-sents the first report of systemic pasteurellosis caused by P .canis in raccoon dogs.关键词
貉/犬巴氏杆菌/分离/生物学特性/16SrRNAKey words
raccoon dog/Pasteurella canis/isolation/physiological characteristic/1 6 S rRNA分类
农业科技引用本文复制引用
芮萍,马增军,郭刚,项方,刘曜综..貉源犬巴氏杆菌的分离与鉴定[J].动物医学进展,2016,(2):125-127,3.基金项目
河北省科技支撑项目(13226609D) （13226609D）
河北省畜牧局计划项目(2011-1-19) （2011-1-19）
