动物营养学报Issue(1)：208-216,9.DOI:10.3969/j.issn.1006-267x.2016.01.027
重组菌丝霉素发酵培养基筛选及重组菌丝霉素在断奶仔猪上的应用
Recombinant Plectasin：Screening of Fermentation Medium and Application for Weaning piglets
摘要
Abstract
The study was aimed to investigate the optimization and screening of fermentation medium for the recombinant plectasin, and the effects of recombinant plectasin on growth performance, nutrient digestibility and intestinal microflora in weaning pigs.In the present study, the liquid fermentation was used to prepare the recombinant plectasin in a 30 L fermenter by using batch-fermentation process, and subsequently the expression levels of recombinant plectasin with low salt, basal glycerin and basal soluble starch mediums were compared. Thirty healthy piglets (Duroc×Landrace×Yorkshire) weaned at 24 d were selected and randomly allotted to five groups according to their initial BW.The groups consisted of control group ( CON group, a basal diet without antibiotic) , sulfuric acid colistin group ( CS group, basal diet+0.3%sulfuric acid colistin) , antimicro-bial peptide group (AP group, basal diet+0.2%recombinant plectasin), probiotics group (PB group, basal diet+0.1%probiotics) and joint application group ( PPB group, basal diet+0.2%recombinant plectasin+0.1%probiotics) .The experiment lasted for 21 days.The results showed that the wet weight of bacteria in low salt group, basal glycerol group and basal soluble starch group reached the highest value at 114 hours of fermenta-tion, which were 450, 402 and 277 g/L, respectively; the concentrations of total protein in supernatant were 0.38, 3.94 and 5.63 g/L, respectively.Animal trial results showed that compared with CON group, average daily feed intake ( ADFI) , average daily gain ( ADG) and ratio of feed to gain ( F/G) were significantly in-creased (P<0.05) and diarrhea incidence tended to decrease in CS and AP groups (0.05<P<0.10).Compared with other groups, the AP group significantly increased the population of Bifidobacteria in ileal digesta ( P<0.05) .Compared with CON group, the other groups tended to increase the apparent digestibility of energy and dry matter (0.05<P<0.10).The apparent digestibility of phosphorus in PB group was significantly greater than that in the rest four groups ( P<0.01) .In conclusion, the yield of recombinant plectasin and the performance of piglets are improved by optimizing the growth medium.And the results preliminarily confirm that recombinant plectasin developed by our laboratory can be used as an alternative to antibiotics in animal feed.关键词
重组菌丝霉素/抗菌肽/生长性能/养分消化率/断奶仔猪Key words
recombinant plectasin/antimicrobial peptide/growth performance/nutrient digestibility/weaning piglets分类
农业科技引用本文复制引用
李延,万津,晨光,陈代文,余冰,何军..重组菌丝霉素发酵培养基筛选及重组菌丝霉素在断奶仔猪上的应用[J].动物营养学报,2016,(1):208-216,9.基金项目
农业部公益性行业科研专项（201403047）；教育部长江学者和创新团队发展计划 （）
