动物营养学报Issue(1)：244-248,5.DOI:10.3969/j.issn.1006-267x.2016.01.031
利用MiSeq测序技术分析锦江牛瘤胃细菌多样性
Analysis of Rumen Bacterial Diversity in Jinjiang Cattle Using MiSeq Sequencing Technology
摘要
Abstract
The present study was designed to reveal the rumen bacterial diversity in Jinjiang cattle using MiSeq high-flux sequencing technology.Ruminal liquid samples were obtained from three Jinjiang cattles ( bull ) weighted (400±20) kg with permanent rumen cannulas.Total DNA was extracted and the 16S rDNA V4 to V5 of bacteria was sequenced by MiSeq sequencing technology.The abundance, distribution and α-diversity index of species were analyzed.The results showed that a total of 56 763 valid 16S rDNA sequences and 946 operational taxonomic unit ( OTU) across 3 samples were obtained.The richness indexes, Chao and Ace inde-xes, of ruminal bacterial were 836 and 841, respectively; and alpha diversity indexes, Shannon and Simpson indexes were 4.96 and 0.021 5, respectively.Fifteen phyla, 23 classes, 26 families and 44 genera were identi-fied via taxonomic summary.The dominant phyla among them were Bacteroidetes ( 64.37%) , Firmicutes (21.20%) and Proteobacteria (7.59%).The most abundant classes were Bacteroidia (52.88%), Clostridia (17.03%), and Prevotellaceae (36.26%).Ruminococcaceae (9.33%) and Lachnospiraceae (4.89%) were the predominant family.At genus level, the high abundance of genera were Prevotella (28.97%), Paraprevo-tella (3.08%), Rikenella (2.28%), Clostridium Ⅳ (1.77%), Succiniclastium (1.62%) and Ruminococcus ( 1.53%) .In conclusion, the diversity of rumen bacterial community in Jinjiang cattle is low.Bacteroidetes is the predominant phylum and Firmicutes is the second most abundant phylum.Prevolla is the most abundant ge-nus in the rumen bacterial community.关键词
锦江牛/瘤胃细菌/多样性/MiSeq测序Key words
Jinjiang cattle/rumenal bacteria/diversity/MiSeq sequencing分类
农业科技引用本文复制引用
高雨飞,欧阳克蕙,瞿明仁,熊小文,温庆琪,许兰娇..利用MiSeq测序技术分析锦江牛瘤胃细菌多样性[J].动物营养学报,2016,(1):244-248,5.基金项目
国家自然科学基金（31260561）；国家现代农业产业技术体系 （）
