动物营养学报Issue(1)：296-302,7.DOI:10.3969/j.issn.1006-267x.2016.01.038
饲粮蛋白质水平对意大利蜜蜂归巢能力及记忆相关基因表达的影响
Effects of Dietary Protein Level on Homing Capability and Memory Related Gene Expression of Apis mellifera ligustica
摘要
Abstract
The objective of this experiment was to explore the effects of dietary protein level on homing capa-bility and memory related gene expression of Apis mellifera ligustica.Nine colonies of local Apis mellifera ligu-stica with equal colony population were randomly divided into 3 groups with 3 replicates in each group.Bees in groups Ⅰ, Ⅱ and Ⅲ were fed diets containing 15%, 25%, 35%protein, respectively.In this study, the homing rate of foragers in the 1 and 2 km away after feeding 45 days and the expression levels of three memory related genes [ glutamate receptor type A gene ( GluRA ) , N-methyl-D-aspartic acid receptor type 1 gene ( Nmdar1) and tyramine receptor type 1 gene ( Tyr1) ] in different day-old ( newly emerged, 10-day-old and 20-day-old) worker bees were detected.The results showed that the homing rates in the 1 and 2 km away in groups Ⅱ and Ⅲwere significantly higher than those in group Ⅰ (P<0.05), while there was no significant difference between groups ⅡandⅢ( P>0.05) .The expression level of GluRA of 10-day-old and 20-day-old worker bees in groupⅢwas significantly higher than that in groupⅠandⅡ( P<0.05) , and that in groupⅡwas significantly higher than that in group Ⅰ ( P<0.05).The expression level of Nmdar1 of 10-day-old and 20-day-old worker bees in groups Ⅱ and Ⅲ was significantly higher than that in group Ⅰ ( P<0.05 ) , but there was no significant difference between groupsⅡ and Ⅲ( P>0.05) .The expression level of Tyr1 of 10-day-old worker bees in groups Ⅱ and Ⅲ was significantly higher than that in group Ⅰ (P<0.05), but there was no significant difference between groups Ⅱ and Ⅲ ( P>0.05).Besides that, there were no significant differences in the expression levels of GluRA, Nmdar1 and Tyr1 of newly emerged worker bees among the three groups (P>0.05), and the expression level of Tyr1 of 10-day-old worker bees among the three groups also had no significant difference ( P>0.05) .In conclusion, the lower level of protein in the diet may infect the homing ability and the expression of memory related genes of Apis mellifera ligustica.关键词
意大利蜜蜂/蛋白质水平/回归率/记忆基因Key words
Apis mellifera ligustica/protein level/homing rate/memory genes分类
农业科技引用本文复制引用
袁安,郭亚惠,吴小波,黄晓,廖春华..饲粮蛋白质水平对意大利蜜蜂归巢能力及记忆相关基因表达的影响[J].动物营养学报,2016,(1):296-302,7.基金项目
国家自然科学基金项目 （）
