单克隆抗体依那西普融合蛋白的活性结构保护OA北大核心CSCDCSTPCD
Active Structure Protection of Monoclonal Antibody Fusion Protein Etanercept†
采用拉伸模拟和伞状采样的方法，利用 Gromacs 软件和 Amber99sb-ildn 分子力场研究了不同浓度的海藻糖体系和海藻糖-甘露醇复合体系中单克隆依那西普融合蛋白二聚体的解离过程。结果表明，海藻糖能显著增强依那西普二聚体活性结构的稳定性，且海藻糖-甘露醇的复合保护优于海藻糖的单一保护；保护剂的种类及其选择性吸附保护的特定位置对依那西普蛋白二聚体活性结构保护效果的影响显著。
The thermodynamic stability of etanercept in different concentrations of the protective agent was in-vestigated. The approaches of umbrella sampling and steered molecular dynamic simulation were adopted to study the dissociation of dimer etanercept with Gromacs software and amber99sb-ildn united atomic force field. The results indicated that the trehalose has ability to enhance the stability of dimer etanercept, and the protec-tive effect of etanercept with …查看全部>>
潘琦;李代禧;郭柏松;杨春生;杨智
上海理工大学食品科学与工程研究所，上海 200093上海理工大学食品科学与工程研究所，上海 200093上海东富龙科技股份有限公司冻干工艺研究室，上海 201108上海东富龙科技股份有限公司冻干工艺研究室，上海 201108上海东富龙科技股份有限公司冻干工艺研究室，上海 201108
化学化工
依那西普拉伸模拟伞状采样海藻糖
EtanerceptSteered molecular dynamicUmbrella samplingTrehalose
《高等学校化学学报》 2016 (2)
纳米微粒增强玻璃化低温保护剂传热性能的研究
274-280,7
国家自然科学基金(批准号:51076108)、上海市自然科学基金(批准号:12ZR1420400)、上海市“创新行动计划”国际科技合作项目(批准号:12430702000)和上海市联盟计划项目资助.? Supported by the National Natural Science Foundation of China( No.51076108), the National Science Foundation of Shanghai, China (No.12ZR1420400), the Innovation Funds for International Cooperation of the Shanghai Committee of Science and Technology, China (No.12430702000) and the Alliance Program in Shanghai, China
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