口腔疾病防治Issue(3)：142-145,4.DOI:10.12016/j.issn.2096-1456.2016.03.003
双向差速法纯化大鼠牙囊细胞
Rat dental follicle cells purification by bidirectional differential method
摘要
Abstract
Objective To develop a simple and fast purifingmethod of dental follicle cell by the character of different velocity of attachment of detachment between dental follicle cells and enamel organ cells. Methods The lower first intact molar germs of SD rats with 5-6 days old were selected and separated. The dental follicle and enamel organ were stipped together under the stereo microscope. After minced into little pieces and degested with trypsin and cultured, the dental follicle cells were purified by diffierential adherence and differential passage. The final purified cells were identified by immunocytochemistry. Results The primay cells were mixed, consisting of dental follice cells and enamel organ cells. After differential adherence and passage, the cells of 2-3th passage became purified dental follicle cells. Purified dental follicle cells were elongate spindle or triangle in shape, positive for vimentin and negative for cytokeratin. Conclusion Bidirectional differential method is an efficient and simple method for the purification of dental follicle cells.关键词
牙囊细胞/纯化/差速贴壁/差速传代/免疫组织化学Key words
Dental follicle cells/Purification/Diffierential adherence/Differential passage/Immunocyto-chemistry分类
生物科学引用本文复制引用
王丽萍,李伯琦,铁晓敏,王琪,刘奕杉..双向差速法纯化大鼠牙囊细胞[J].口腔疾病防治,2016,(3):142-145,4.基金项目
国家自然科学基金(81560178) （81560178）
