口腔疾病防治Issue(3)：146-149,4.DOI:10.12016/j.issn.2096-1456.2016.03.004
TNF-α诱导后的小鼠成骨细胞对破骨前体细胞增殖的影响
The influence of the TNF-αinduced mouse osteoblast on the proliferation of osteoclast precursor cells
摘要
Abstract
Objective To study the influence of the TNF-α induced mouse osteoblast under the inflammation condi-tion on the function of the proliferation of osteoclast precursor cells by the indirect co-culture of the mouse osteoblast and osteoclast precursor cells. Methods The mouse osteoblast were cultured for 2, 4, 8, 16 h respectively according to the TNF-α10 ng/mL concentration in order to be induced into inflammatory state. Its supernatant was extracted respectively and co-cultured with mouse monocyte with the addition of 30 μg/L activator for nuclear factor- receptor nuclear factor kappa ligand ( RANKL) . The proliferation activity was detected by laser scanning confocal microscopy and cell counting kit-8 cell ( CCK-8) method. Results When the supernatant induced by TNF-α for 2 h was co-cultured with monocyte with RANKL, there was no significant difference between the experimental group and the control group. The proliferation of primary osteoclast in experimental group was significant higher than control group when the osteoblast was induced at 4 h. However, the proliferation of primary osteoclast in experimental group was significant lower than control group when the osteoblast was induced at 8, 16 h. Conclusion The osteoblasts may promote the proliferation of osteoclast precursor cells under transient inflammation stimulation. However, the osteoblast may have inhibitory effects on the proliferation of osteoclast precursor cells under the prolonged inflammatory stimulation.关键词
肿瘤坏死因子-α/成骨细胞/单核细胞/共培养/炎症Key words
Tumour necrosis factor-α/Osteoblast/Monocyte/Co-culture/Inflammatory分类
医药卫生引用本文复制引用
刘航航,何武林,魏叶红,陈建伟,文军,杨烁,杨格..TNF-α诱导后的小鼠成骨细胞对破骨前体细胞增殖的影响[J].口腔疾病防治,2016,(3):146-149,4.基金项目
国家自然科学基金(81300905) （81300905）
广东省医学科学技术研究基金(B2014035) （B2014035）
