国际医药卫生导报2016，Vol.22Issue(3)：300-302,3.DOI:10.3760/cma.j.issn.1007-1245.2016.03.002
应用双向电泳分析结核性脑膜炎与隐球菌性脑膜炎脑脊液的蛋白差异
The analysis of protein differences in cerebrospinal fluid of tuberculous meningitis and cryptococcus meningitis by two-dimensional electrophoresis
摘要
Abstract
Objective To screen the diagnostic markers between tuberculous meningitis and cryptococcus meningitis by analyzing the protein differences in cerebrospinal fluid (CSF).Methods The proteins in CSF of 4 cases of tuberculous meningitis and 4 cases of cryptococcal meningitis were separated by two-dimensional gel electrophoresis (2-DE).The differential proteins expression was descriptively analyzed according to the protein isoelectric point PI,molecular weight,matching rate,gray value by PDQuest8.0 software.Results 111 and 114 protein spots were captured from CSF of tuberculose meningitis and cryptococcus meningitis.The molecular weight of proteins ranged from 10 to 130 kDa.The isoelectric point (PI) was from 4.4 to 6.7.The matching rate of two groups was 95％.There were 24 differential proteins between two groups,of which 3 proteins were expressed only in tuberculous meningitis and 2 proteins expressed only in cryptococcal meningitis.19 matching protein point were two-fold differential expression.Conclusion 2-DE profiles with high resolution and reproducibility of CSF for tuberculous meningitis and cryptococcus meningitis are established,and some differences in protein expression exist between the two.关键词
双向电泳/结核性脑膜炎/隐球菌性脑膜炎/脑脊液Key words
Two dimension electrophoresis/Tuberculous meningitis/Cryptococcus meningitis/Cerebrospinal fluid引用本文复制引用
黄绍梅,杨瑜,邱薇,吴睿彦,谢贝,刘志辉,陈志成..应用双向电泳分析结核性脑膜炎与隐球菌性脑膜炎脑脊液的蛋白差异[J].国际医药卫生导报,2016,22(3):300-302,3.基金项目
广东省科技计划项目(2012A030400034) （2012A030400034）
广州市科技计划项目(2014Y2-00117) （2014Y2-00117）
Science and Technology Program of Guangdong,China (2012A030400034) （2012A030400034）
Science and Technology Program of Guangzhou,China (2014Y2-00117) （2014Y2-00117）
