临床与病理杂志Issue(1)：61-66,6.DOI:10.3978/j.issn.2095-6959.2016.01.011
ALA-PDT对尖锐湿疣凋亡及细胞因子的影响
The effect of ALA-PDT of apoptosis and cytokine in the patients with cindyloma acuminate
摘要
Abstract
Objective:To detect the expression of the apoptosis related factors (Fas, caspase3, Bcl-2) and cytokines (CCL-20, TNF-α, IL-1) in patients with condyloma acuminatum before and atfer the treatment with ALA-PDT, and explore the mechanism of ALA-PDT in the treatment of condyloma acuminatum.Methods:A total of 10 patients who were diagnosed with condyloma acuminatum were enrolled in this study and were treated by ALA-PDT for 3 or 4 times. hTe lesions of condyloma acuminatum before and atfer the treatment (normal healthy skin tissues as the control), were collected. hTe expression of Fas, caspase3, Bcl-2, CCL-20, TNF-α, IL-1 mRNA with Fluorescene quantitative reverse transcription polymerase chain reaction as well as the expression of Fas, caspase3, Bcl-2, CCL-20, TNF-α, IL-1 with Western blot were conducted.Results: The expression of Fas, caspase3, Bcl-2, CCL-20, TNF-α, IL-1 in condyloma acuminatum was higher than those in the normal skin tissues (P<0.05). Compared with before treatment with ALA-PDT, the expression of Bcl-2, CCL-20, TNF-α, IL-1 was signiifcantly decreased atfer the treatment with ALA-PDT (P<0.01), whereas the Fas, caspase3 expression was signiifcantly increased (P<0.01). Conclusion: ALA-PDT is an effective treatment for condyloma acuminatum. It can promote cell apoptosis in condyloma acuminatum by including cell surface apoptosis receptor Fas, caspase3 expression and inhibiting apoptosis inhibitory factor Bcl-2 expression. The specific apoptosis pathways include mitochondrion-mediated apoptosis (intrinsic pathway) and death receptor-mediated apoptosis (extrinsic pathway). And it possibly plays a local immunomodulatory effect in condyloma acuminatum through inhibition of proinlfammatory cytokines CCL-20, TNF-α and IL-1.关键词
尖锐湿疣/ALA-PDT/凋亡/细胞因子Key words
condyloma acuminatum/ALA-PDT/apoptosis/cytokine引用本文复制引用
胡永轩,韩雪,谭妙娟,周冼苡,吴中,毛祖豪,胡燕卿,黄诗韵,刘康兴,卢艳..ALA-PDT对尖锐湿疣凋亡及细胞因子的影响[J].临床与病理杂志,2016,(1):61-66,6.基金项目
广州市天河区科技计划资助(201404KW007)；广东省自然科学基金资助(2015A030310035)。Tis work was supported by Tianhe Science and Technology Project Foundation (201404KW007) and Guangdong Natural Science Foundation (2015A030310035), P. R. China （201404KW007）
