广西医学Issue(1)：10-13,4.DOI:10.11675/j.issn.0253-4304.2016.01.03
缺血性损伤致薄型子宫内膜动物模型的建立
Animal modeling of thin endometrium caused by ischemic injury
摘要
Abstract
Objective To establish the animal model of thin endometrium based on ischemic injury .Methods Thirty SD rats were divided into control group ,modelⅠgroup and model Ⅱgroup,with 10 rats in each group.The bilateral arteriae ovarica occlusion was performed in the model Ⅰgroup and model Ⅱgroup for 20 and 30 minutes respectively ,and then the blood flow reperfused .The arteriae ovarica occlusion was not performed in the control group .After 5 days, the pathological changes of endometrial tissues , expression of endometrial microvessel density ( MVD) ,areas of vimentin and keratin in unit endometrial areas were observed in three groups .Results Compared to control group,model Ⅰgroup and model Ⅱ group had thinner endometrial thickness (P<0.05),less endometrial glands which appeared sparse arrangement and developmental delay ,lower expression of MVD,and less areas of vimentin and keratin(P<0.05). There were no significant differences in the indices mentioned above between two model groups (P>0.05).Conclusion The ischemic injury-induced animal model of thin endometrium can be established by a 20-minute arteriae ovarica occlusion.This model has good repeatability, and accorded with the requirement of establishing an animal model simply and rapidly which is more similar to human pathological state .关键词
薄型子宫内膜/缺血性损伤/动物模型/卵巢动脉/大鼠Key words
Thin endometrium/Ischemic injury/Animal model/Arteriae ovarica/Rat分类
医药卫生引用本文复制引用
李楠,陈梅,李小宁,孙康..缺血性损伤致薄型子宫内膜动物模型的建立[J].广西医学,2016,(1):10-13,4.基金项目
陕西省科技厅自然科学基础研究项目 （）
