摘要
Abstract
The impurities of chloroquine phosphate in Compound bendazol and hydrochlorothiazide capsules, were identi⁃fied using high performance liquid chromatography(UV detector and two diode array detector)and mass spectrometry. Us⁃ing Luna C18 column with the mobile phase of methanol and acetic acid-2%,(υ/υ10∶90), with flow rate of 0.5 ml/min, col⁃umn temperature at room temperature,the injection volume was 20μl, the UV detection wavelength was 324nm and the DAD detection wavelength range was 200~400nm, by high performance liquid chromatography. In mass spectrometry, ESI electro⁃spray ionization was used, atomization gas pressure was 45 psi, with drying air flow rate 10 L/min and temperature 350℃and capillary voltage 3.5 kV, scaning MS. And then the second scan was carried out to the first target ion with voltage 20eV and collection range of 100-1000 m/z. UV detection results showed that the impurity peak in Compound bendazol and hydro⁃chlorothiazide capsules was similar to that in chloroquine phosphate. And the DAD detection result of the impuritie was simi⁃lar to chloroquine. The second mass scan result showed that the molecular fragments were similar to chloroquine. The impuri⁃ties generated by chloroquine phosphate in Compound bendazol and hydrochlorothiazide capsules is N-deethylation chloro⁃quine.关键词
复方地巴唑氢氯噻嗪胶囊/磷酸氯喹/HPLC/HPLC-MSKey words
compound bendazol and hydrochlorothiazide capsules/chloroquine phosphate/HPLC/HPLC-MS分类
医药卫生
