环境与可持续发展Issue(1)：158-160,3.
荧光法测定悬浮培养藻体叶绿素含量的研究
Study about Chlorophyll Fluorescence Determination with the Aalgae Suspension Culture
崔建升 1樊琳琳 1段莉丽 1高柳堂 1高思 1周盼 1张乃琦 2潘少伟3
作者信息
- 1. 河北科技大学环境科学与工程学院，石家庄 050000
- 2. 河北省交通规划设计院，石家庄 050000
- 3. 河北升泰环境检测有限责任公司，石家庄 050000
- 折叠
摘要
Abstract
Laboratory always obtain the content of algae volume by measuring the chlorophyll a fluorescence intensity. According to the settlement of algae cells, fluorescence value are greatly influenced by static duration, when the static duration is 1min, attenuation rate of fluorescence was ΔF/F0=-9�96% and the determination of algae density is not exact. In the article, a method of algae culture preparation was established by adding agar powder(0�08~0�10g/100mL) to the culture. The suspensibility and stability of the culture was enhanced dramatically by this method, after standing 1 hour, attenuation rate of fluorescence was ΔF/F0=-4�82%≤± 5%�The method enhance the accuracy of chlorophyll contents measurement by chlorophyll spectrofluorimetry and reduces the effect of culture sedimentation to the measurement.关键词
藻液叶绿素荧光测定/荧光光度法/悬浮稳定剂Key words
Algal chlorophyll fluorescence determination/spectrofluorimetry/Suspending stabilizer分类
资源环境引用本文复制引用
崔建升,樊琳琳,段莉丽,高柳堂,高思,周盼,张乃琦,潘少伟..荧光法测定悬浮培养藻体叶绿素含量的研究[J].环境与可持续发展,2016,(1):158-160,3.
