湖南中医药大学学报2016，Vol.36Issue(2)：40-43,4.DOI:10.3969/j.issn.1674-070X.2016.02.011
银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究
Experimental Research of Yinhuang Qingfei Capsules Containing Serum on the Respiratory Syncytial Virus in Vitro
摘要
Abstract
Objective To study Yinhuang Qingfei capsules containing serum on the respiratory syncytial virus in vitro. Methods The cytopathic effect (CPE) and optical density (OD) of respiratory syncytial virus (RSV) infection Hep-2 cells, which were intervened by Yinhuang Qingfei capsules, were by CPE and MTT methods. The inhibition effects of Yinhuang Qingfei capsules on RSV with different doses and different administration methods (drug prevention, treatment, directly kill) were observed. Results Compared with the corresponding concentration of blank serum, the serum OD values in Yinhuang Qingfei capsule and ribavirin tablets containing serum groups were significantly increased (P<0.05); Compared with other oncertrations of the same groups, the OD valiues of ribavirin with 3.125% dilution and Yinhuang Qingfei with 6.125% dilution were increased obviuosly (P<0.05). Conclusion Yinhuang Qingfei capsule showed directly inactivation effect on RSV, blocking effect on RSV invade Hep-2 cells, proliferation inhibition on RSV in Hep-2 cell, and the ribavirin with 3.125% dilution and Yinhuang Qingfei with 6. 25% dilution showed strongest anti-RSV effect in vitro.关键词
呼吸道合胞病毒/银黄清肺胶囊/含药血清/抗病毒/葶苈子/炙麻黄/苦杏仁Key words
respiratory syncytial virus/Yinhuang Qingfei capsule/drug serum/antiviral/pepperweed seed/prepared ephedra/bitter almond kernel分类
医药卫生引用本文复制引用
彭志,卢芳国,屈金艳,李玲,范伏元,那婧婧,李湘兰..银黄清肺胶囊含药血清体外抗呼吸道合胞病毒实验研究[J].湖南中医药大学学报,2016,36(2):40-43,4.基金项目
国家自然基金课题(81473468,81072751) （81473468,81072751）
湖南省自然科学基金项目(13JJ6062) （13JJ6062）
湖南省教育厅科学研究重点项目(13A066) （13A066）
湖南省高校科技创新团队"感染性疾病中医药防治研究"资助项目 （）
湖南省战略性新兴产业科技攻关类项目(2014CK1022). （2014CK1022）
