湖南中医药大学学报2016，Vol.36Issue(2)：54-57,4.DOI:10.3969/j.issn.1674-070X.2016.02.015
电针内关、百会穴对脑缺血/再灌注大鼠GRP78和Caspase-12基因表达的影响
Effect of Electroacupuncture at Neiguan and Baihui Points on GRP78 and Caspase-12 Gene Expression in Rats with Ischemia-Reperfusion Injury
摘要
Abstract
Objective To observe the effects of electro-acupuncture (EA) at Neiguan and Baihui point on GRP78 and Caspase-12 gene expression in rats with ischemia/ reperfusion (IR) injury and investigate protective effects of acupuncture wheather related to endocytoplasmic reticulum (ER) stressapoptosis passage. Methods 100 rats were randomly assigned to five groups, 20 in each group: the normal group, sham-operation group, operation model group, edaravone group and EA group. The IR model of middle cerebral artery occlusion (MCAO) was established by suture embolic method. The apoptosis index of nerve cells in rats were measured by TUNEL staining method. The mRNA expression of GRP78 and Caspase-12 were measure by Real-time polymerase chain reaction (RT-PCR). Results Compared with normal group and sham-operation group, the apoptosis indexes and mRNA expression of GRP78 and Caspase-12 in operation group, Edaravone group and EA group were increased, with statistical significance (P<0.05 or P<0.01); Compared with operation model group, the apoptosis indexes and Caspase-12 mRNA expression in edaravone group and EA group were decreased (P<0.01), GRP78 mRNA expression were increased obviously (P<0.01); there were no significant difference between edaravone group and EA group on the above indexes (P>0.05). Conclusion Acupuncture at Neiguan and Baihui points could effectively suppress the nerve cell apoptosis in cerebral ischemia. The underlying mechanism might be related to upregulation of the ERS-protective GRP78 expression and inhibition of apoptosis-promotion Caspase-12 expression.关键词
细胞凋亡/电针/脑缺血/再灌注损伤/百会/内关/GRP78/Caspase-12Key words
cell apoptosis/electroacupuncture/cerebral ischemia/reperfusion injury/Baihui point/Neiguan point/GRP78/Caspase-12分类
医药卫生引用本文复制引用
沈菁,雷晓明,宋洋,谭杏,刘琴,戴丽雯,李文慧,郁洁..电针内关、百会穴对脑缺血/再灌注大鼠GRP78和Caspase-12基因表达的影响[J].湖南中医药大学学报,2016,36(2):54-57,4.基金项目
国家自然科学基金青年项目(81202771) （81202771）
教育部新教师类项目(20114323120005) （20114323120005）
针灸推拿湖南省重点学科资助. （）
