军事医学Issue(1)：40-44,5.DOI:10.7644/j.issn.1674-9960.2016.01.010
猪链球菌溶血素（SLY）引起血小板聚集活性分析
Activity identification of Streptococcus suis suilysin inducing platelets aggregation
摘要
Abstract
Objective To explore the interaction of streptococcus suis serotype 2 recombinant suilysin ( SLY ) with platelets, and provide the theoretical basis for clinic treatment of patients infected with S.suis.Methods The nickel column affinity chromatography was used to purify the recombinant SLY.The hemolytic acivity was identified by optical density before the platelets aggregation induced by a SLY was detected by a platelet aggregometer or electron microscope and the effect of aspirin on platelets aggregation was analyzed.The impact of wild type 05ZYH33 and sly-deficient mutant strainΔSLY on platelets of mice was compared to predict the interaction of the SLY with platelets in vivo.Results and Conclusion Hemolytic activity of recombinant SLY was 2000 hemolytic units( HU) and platelets aggregation was induced at 1 μg/ml.The aggregation can be inhibited by aspirin in 5 mmol/L.SLY can also increase the volume and reduce the amount of platelets in mice.关键词
2型猪链球菌/猪链球菌溶血素/血小板聚集/阿司匹林/血小板减少Key words
Streptococcus suis serotype 2/suilysin/platelets aggregation/aspirin/thrombocytopania分类
医药卫生引用本文复制引用
张省委,刘鹏,徐茂凯,尚学义,郑玉玲,袁媛,姜永强..猪链球菌溶血素（SLY）引起血小板聚集活性分析[J].军事医学,2016,(1):40-44,5.基金项目
国家重点基础研究发展计划资助项目 （）
