军事医学Issue(1)：51-54,4.DOI:10.7644/j.issn.1674-9960.2016.01.012
慢病毒介导的敲低E6 AP表达抑制胃癌细胞增殖和迁移
Lentivirus-mediated knockdown of human E6 AP inhibits gastric cancer cell prolifera-tion and migration
摘要
Abstract
Objective To detect the effect of E6AP on gastric cancer cell proliferation and migration.Methods The expression of E6AP in different gastric cancer cell lines and normal gastric mucosa epithelial cell lines was detected by Western blotting.Gastric cancer cells BGC-823 stably expressing E6AP short hairpan RNA(shRNA) were obtained by lentiviral vector of E6AP.The effect of E6AP on BGC-823 cell growth and migration was determined by CCK-8 kit, Tran-swell and wound healing assay.Results Gastric cancer cell line BGC-823 in which E6AP was stably knocked down was established.Knockdown of E6AP inhibited the proliferation and migration of BGC-823 cells.Conclusion E6AP plays a key role in gastric cancer proliferation and migration.关键词
E6AP/慢病毒属/RNA干扰/胃肿瘤/细胞增殖Key words
E6AP/lentivirus/RNA interference/gastric neoplasms/cell proliferation分类
医药卫生引用本文复制引用
熊加秀,卫勃,陈志达,张亚楠,贾小萌,陈滢洁,熊柚花,丁丽华,叶棋浓,刘文忠..慢病毒介导的敲低E6 AP表达抑制胃癌细胞增殖和迁移[J].军事医学,2016,(1):51-54,4.基金项目
国家自然科学基金资助项目（81101883，81272231，31470773）；北京市科技新星计划资助项目 （）
