临床输血与检验Issue(1)：9-13,5.DOI:10.3969/j.issn.1671-2587.2016.01.003
Th17/Treg细胞在子痫前期患者外周血及胎盘组织中的表达水平
Expressions of Th17 Cells and Treg Cells Cytokines in Preeclampsia
张世芬 1张颖新 1徐军 2周继 1张军辉 1谢红霞1
作者信息
- 1. 230001 安徽省妇幼保健院
- 2. 解放军105医院病理科
- 折叠
摘要
Abstract
Objective To observe the cytokine expressions of Th17 and Treg cells in peripheral blood and the presence of IL-17A and FoxP3 positive cells in decidua with preeclampsia(PE) patients in order to reveal the role of Th17/Treg cells in PE patients.Methods Select20 cases with PE and20 cases of healthy women with late pregnancy were selected. Flow cytometry was used to analyze the change of CD4+IL-17A+ and CD4+CD25+ FoxP3+ cells, and qRT-PCR was used to detect the expression of RORγt mRNA of Th17,cells and FoxP3 mRNA of Treg cells in peripheral blood. Decidual tissue was collected in uterus wall placenta and immunohistochemical staining was used to detect the expression of IL-17 and specific transcription factor Foxp3 of decidual CD4+CD25+ regulatory cells.Result Circulating Th17 frequencies (CD4+IL17+/CD4+T cells) were markedly higher in patients with PE (2.96±0.28%) than those with normal gravida with late pregnancy (1.61±0.37%) (P <0.05). However, CD4+CD25+Foxp3+ Tregs in CD4+ cells group in peripheral blood of PE patients(2.31±0.54%) were significantly lower than those in normal gravidas (3.86±0.32%,P<0.05). Simultaneously, Treg cell frequencies of normal early pregnant women were increased compared with control(P<0.05). The expression of RORγt mRNA in peripheral blood of PE patients was significantly higher than that in normal control group, while the expression of FoxP3 mRNA in peripheral blood of PE patients was markedly lower than that in normal control group(P <0.05). Additionally, the positive expression of IL-17A in decidua of PE patients was noted, while slight expression was seen in normal pregnancy.Conclusion The number of Th17 cells was increased whereas Treg cells were decreased in PE patients. The imbalance of Th17 and Treg cells may play an important role in the immune system in the pathophysiology of preeclampsia.关键词
子痫前期/Th17细胞/Treg细胞Key words
Preeclampsia/Th17 cells/Treg cells分类
医药卫生引用本文复制引用
张世芬,张颖新,徐军,周继,张军辉,谢红霞..Th17/Treg细胞在子痫前期患者外周血及胎盘组织中的表达水平[J].临床输血与检验,2016,(1):9-13,5.
