农药学学报Issue(1)：49-56,8.DOI:10.16801/j.issn.1008-7303.2016.0005
氟虫腈与昆虫γ-氨基丁酸受体相互作用研究
Interactions between the fipronil and the insect γ-aminobutyric acid receptor
摘要
Abstract
Herein the fluorescent-labeled ligand and the molecular simulation method were used to study the interactions between the γ-aminobutyric acid (GABA) receptor of the insects and fipronil. The fluorescence labeling experiment results showed that strong interactions were existed between fipronil and the GABA receptor in the brain of the housefly. The maximum number of binding site [RT] and the equilibrium dissociation constants (Kd) were (21.3±2.5) pmol/mg protein and (109±9) nmol/L, respectively. Furthermore, the molecular simulation results also suggested that there were some strong hydrogen bond interactions between fipronil and the RDL receptor of the fruit fly. In addition, the CDOCKER interaction energy between them was –137.93 kJ/ mol. The above results clearly illustrated that the fipronil has strong affinity on the GABA receptor of insects and high toxicity to insects.关键词
氟虫腈/昆虫/γ-氨基丁酸受体/分子模拟/荧光标记Key words
fipronil/insect/γ-aminobutyric acid receptor/molecular simulation/fluorescence labeling分类
农业科技引用本文复制引用
张元,张博,任天瑞..氟虫腈与昆虫γ-氨基丁酸受体相互作用研究[J].农药学学报,2016,(1):49-56,8.基金项目
国家自然科学基金资助项目(21172147) （21172147）
