曲阜师范大学学报（自然科学版）Issue(1)：97-101,5.DOI:10.3969/j.issn.1001-5337.2016.1.097
重组人β防御素-3表达条件的优化及其对抑菌活性的影响
Optimization of the Expression of Recombinant Human Beta Defensin-3 in Pichia Pastoris and Its Effect on Antibacterial Activity
摘要
Abstract
To study the effect on expression level and antibacterial activity of recombinant human beta defensin-3 (rhβD-3)by Pichia pastoris ,the requirements for the flask-shaking culture fermentation,such as pH,culture temperature,methanol concentration and so on,were optimized.The results showed that the optimal expression conditions were as follows:the pH value of the culturing BMMY medium was 6.0, medium volume was 50 mL/500 mL,the yeast was cultured under 28 ℃,290 r/min for 48 hours,and the methanol concentration was 0.5% per 24 hours.It was found that the expression level of rhβD-3 could be increased into 340 μg/mL and the antibacterial activity of rhβD-3 could also be increased.关键词
重组人β防御素-3/摇瓶发酵/表达优化Key words
recombinant human beta defensin-3/flask-shaking fermentation/optimization of expression分类
生物科学引用本文复制引用
高飞,郭春晓..重组人β防御素-3表达条件的优化及其对抑菌活性的影响[J].曲阜师范大学学报（自然科学版）,2016,(1):97-101,5.基金项目
山东省高等学校科技计划项目（J13LE14）． （）
