曲阜师范大学学报（自然科学版）Issue(1)：102-104,3.DOI:10.3969/j.issn.1001-5337.2016.1.102
阿式肠杆菌和萎缩芽孢杆菌扫描电镜样品制备观察和分析
Preparation for Observation and Analysis of Enterobacter Asburiae and Bacillus Atrophaeus SEM Specimen
李修善1
作者信息
- 1. 曲阜师范大学生命科学学院，273165，山东省曲阜市
- 折叠
摘要
Abstract
To get high-resolution and shape integral graphics of Enterobacter Asburiae and Bacillus At-rophaeus using JSM-6700F.The method is that,after acquisition of pure microorganism by precisely control the culture condition in media,we prepared the sample with double fixation,phosphate buffer solution rinse,acetone dehydration,and multiple surface platinum plating.High-resolution and shape integral graphics were achieved using SEM after the preparation.The micororganism were in richmorphological di-versity and stereoscopic impression.Our results would be benefit to other researchers using SEM.关键词
阿式肠杆菌和萎缩芽孢杆菌/扫描电镜/样品制备/观察/图像分析Key words
Enterobacter Asburiae and Bacillus Atrophaeus/scanning electron microscope (SEM)/specimen preparation/observation/image analysis分类
生物科学引用本文复制引用
李修善..阿式肠杆菌和萎缩芽孢杆菌扫描电镜样品制备观察和分析[J].曲阜师范大学学报（自然科学版）,2016,(1):102-104,3.
