山东医药Issue(4)：5-7,3.DOI:10.3969/j.issn.1002-266X.2016.04.002
黄芩素联合两性霉素B对烟曲霉菌生物被膜的体外作用
Effects of baicalein combined with amphotericin B on aspergillus fumigatus biofilm in vitro
摘要
Abstract
Objective To investigate the destructive effect and sterilizing effect of baicalein combined with amphoteri-cin B ( AMB) on aspergillus fumigatus biofilm. Methods The broth microdilution method was employed to test the mini-mum inhibitory concentration ( MIC) and minimum fungicidal concentration ( MFC) of baicalein and AMB, and the biofilm models of aspergillus fumigatus were established, and the models were divided into the blank group, baicalein group, AMB group and baicalein+AMB group. After modeling for 24 hours, the corresponding drugs were added to the baicalein group, AMB group and baicalein+AMB group. After 48-hour intervention, crystal violet assay was operated for the semi-quantifi-cation and extracellular matrix was used to observe the biofilm morphology under fluorescence microscopy. Results Crystal violet semi-quantification indicated that there was no significantly difference between AMB group and the control group ( P>0. 05), the biofilm of the baicalein group was significantly lower than that of either AMB group or the control group (P<0. 05), and the baicalein+AMB group was lower than the baicalein group (P<0. 05). Fluorescence microscopy showed that the hypha of control group and AMB group was crossed and encoded with rich extracellular matrix, the hypha of the ba-icalein group was still intensively crossed and became smoother and slender with less extracellular matrix, in the baicalein+AMB group, mycelium was significantly reduced with hardly extracellular matrix. Conclusions Baicalein can destroy the extracellular matrix of aspergillus fumigatus biofilm. Baicalein combined with AMB can increase the penetration of AMB on aspergillus fumigatus and improve its sterilizing effect.关键词
烟曲霉/生物被膜/黄芩素/两性霉素BKey words
aspergillus fumigatus/biofilm/baicalein/amphotericin B分类
医药卫生引用本文复制引用
李冰,陈一强,孔晋亮,罗劲,董必英..黄芩素联合两性霉素B对烟曲霉菌生物被膜的体外作用[J].山东医药,2016,(4):5-7,3.基金项目
国家自然科学基金资助项目(81260002)。 （81260002）
