山东医药Issue(5)：19-22,4.DOI:10.3969/j.issn.1002-266X.2016.05.007
替莫唑胺耐药细胞系 SHG-44／TR 生物学特征及其耐药机制
Biological characteristics and resistance mechanism of temozolomide resistance cell line SHG-44/TR
摘要
Abstract
Objective To investigate the biological characteristics and resistance mechanism of human glioma cell line SHG-44/TR which is resistant to temozolomide ( TMZ) .Methods We induced the human glioma SHG-44 to be re-sistant to TMZ by dose escalation in vitro, and then established the drug-resistant cell line SHG-44/TR.The colony forma-tion assay and MTT colorimetric assay was used to measure the proliferation, resistance and resistance index of SHG-44/TR cells;Western blotting was used to detect the expression changes of resistance protein O6-methylguanine-DNA methyl trans-ferase (MGMT) and human mismatch repair protein 2 (hMSH2);and flow cytometry was used to detect the SHG-44/TR cell cycle.Results After eight months, we successfully established TMZ-resistant glioma cells SHG-44/TR.Significant difference was found in the TMZ-resistance between SHG-44 and SHG-44/TR (P<0.05).SHG-44/TR had no cell cycle arrest phenomenon.The expression of MGMT protein did not change in SHG-44/TR cells as compared with that of parent cells, but the expression of hMSH2 was significantly decreased (P<0.05).Conclusions The hMSH2 expression of TMZ-resistant glioma cells SHG-44/TR is decreased, and the mechanism may be DNA mismatch repair missing.关键词
替莫唑胺/耐药机制/脑胶质瘤/错配修复/O6-甲基鸟嘌呤-DNA甲基转移酶Key words
temozolomide/drug-resistance mechanism/glioma/mismatch repair/O6-methylguanine-DNA methyl-transferase分类
医药卫生引用本文复制引用
丁凤霞,朱仲玲,阎昭..替莫唑胺耐药细胞系 SHG-44／TR 生物学特征及其耐药机制[J].山东医药,2016,(5):19-22,4.基金项目
“十二五”重大新药创制科技重大专项课题（2013 ZX09303001）；国家自然科学基金项目（81402481）；天津卫生局科技基金项目（2014KZ085）。 （）
