天津中医药Issue(1)：35-38,4.DOI:10.11656/j.issn.1672-1519.2016.01.09
电针对睡眠剥夺大鼠行为学和脾脏IL-1β、IL-6和TNF-α的影响
Effects of electro-acupuncture on behavior change and cytokines IL-1β, IL-6, TNF-αin the spleen of sleep deprivation rats
胡天俊 1虞洁 1王秀莲 1何扬子2
作者信息
- 1. 暨南大学，广州 510630
- 2. 暨南大学附属第一医院，广州 510630
- 折叠
摘要
Abstract
Objective] To observe the effect of electro-acupuncture on behavior change and the cytokines of IL-1β, IL-6, TNF-αin the spleen of sleep deprivation rats to investigate the mechanism of electro-acupuncture treatment in insomnia. [Methods] Thirty-two Wistar rats with SPF level were randomized into the control group, model group, diazepam group and electro-acupuncture group. The sleep deprivation rats were established by DL-P-Chlorophenylalanine (PCPA) abdominal injection. The diazepam group were treated by diazepam, and the electro-acupuncture group were treated by electro-acupuncture with Baihui (GV20) and Shenting (GV24) acupoints. Behavior change and the cytokines of sleep deprivation rats were observed and analyzed by real time-PCR after 5 days continuously treatment. [Results] Comparison with the control group in the open field test, the crossing scores, the rearing scores and the pace time in center of the rats were significantly up-regulated in the model group (P<0.05). On the other hand, all of those scores mentioned above were significantly increased in the group of diazepam and electro-acupuncture (P<0.05). In the results of real time-PCR, the IL-1βand the TNF-αexpression levels were remarkably higher in the model group than in the control group (P<0.01). Furthermore, the IL-1βand the TNF-αexpression levels in the diazepam group and the electro-acupuncture group were Significantly down-regulated (P<0.05). But there was no obviously results in each group with the level of IL-6(P>0.05). [Conclusion] The electro-acupuncture can effectively treat the symptoms of Insomnia, and it also can proper control the level of cytokines.关键词
电针/睡眠剥夺/细胞因子Key words
electro-acupuncture/sleep deprivation/cytokine分类
医药卫生引用本文复制引用
胡天俊,虞洁,王秀莲,何扬子..电针对睡眠剥夺大鼠行为学和脾脏IL-1β、IL-6和TNF-α的影响[J].天津中医药,2016,(1):35-38,4.
