天津中医药Issue(2)：90-95,6.DOI:10.11656/j.issn.1672-1519.2016.02.08
冠状动脉结扎制备大鼠心肌梗死模型及评价实验研究
Establishment and evaluation experimental study of acute myocardial infarction in rats
摘要
Abstract
Objective] To explore the establishment of a stable and reliable, easy operation and high survival rates of rats with acute myocardial infarction model preparation and effective evaluation methods. [Methods] The 50 male SD rats were anesthetized, thoracotomy left anterior descending coronary artery ligation. After 24 h, observed with echocardiograph changes in left ventricular function and left ventricular catheterization rat blood dynamics. Remove the heart, TTC sections were stained; automatic biochemical analyzer to detect blood serum creatine kinase indicators. [Results] This method of surgery survival rates as high as 91.67%. After 24 h, remove the cardiac left ventricle infarcted ventricular wall visible color of light than the surrounding myocardium. In the model group, echocardiographic and hemodynamic showed left ventricular function and wall motion was obviously weaker than the sham group. TTC staining showed significant myocardial infarction in the model group. [Conclusion] This method improves the survival rate of rats with acute myocardial infarction model, the model is stable, reproducible, and can meet once ligation or ischemia-reperfusion needs.关键词
急性心肌梗死/动物模型/超声心动图/血流动力学Key words
acute myocardial infarction/animal model/echocardiography/hemodynamic分类
医药卫生引用本文复制引用
胡珍,陈景瑞,魏静,李澜,王玲燕,樊官伟..冠状动脉结扎制备大鼠心肌梗死模型及评价实验研究[J].天津中医药,2016,(2):90-95,6.基金项目
国家重点基础研究发展计划（973计划）项目（2012CB518404）；国家自然科学基金资助项目（81273891）。 （）
