天津中医药Issue(2)：96-99,4.DOI:10.11656/j.issn.1672-1519.2016.02.09
伸筋易骨矫形手法对兔膝关节软骨细胞代谢及 Sox9表达的影响
Effects of Shenjin Yigu orthopaedic manipulation on chondrocytes metabolism and the expression of Sox9 in rabbits
摘要
Abstract
Objective] To study the effects of Shenjin Yigu orthopaedic manipulation on rabbit chondrocytes metabolism and the expression of Sox9. [Methods] KOA rabbits were randomly divided into 2 groups. The treatment group used Shenjin Yigu orthopaedic manipulation therapy, the control group using intra-articular injection of sodium hyaluronate, culturing chondrocytes in vitro after drawing materials. Microscope observation and comparing of chondrocytes’ proliferation between two groups by MMT method, analyzing the expression of Sox9 of chondrocytes between two group by Western-blot method. [Results] Microscopic observation of the treatment group and control group of chondrocytes proliferation faster than those in the blank group and the multiplicaion rate of the treatment group was fastest than those in the control and blank groups, and better proliferation of chondrocytes in the treatment group than that in the control group detected by MMT method (P<0.05). Western blot analysis showed that the expression of Sox9 was higher in the treatment group than that in the control group (P<0.05). [Conclusion] Shenjin Yigu orthopaedic manipulation can improve the speed of proliferation of chondrocytes, enhancing chondrocytes’ multiplication ability. Simultaneously this manipulation can increase the expression level of Sox9 in injured cartilage tissue, to promote the synthesis of chondrocyte metabolism ability.关键词
伸筋易骨矫形手法/膝关节/软骨细胞代谢/Sox9Key words
Shenjin Yigu orthopaedic manipulation/knee joint/chondrocyte metabolism/Sox9分类
医药卫生引用本文复制引用
赵强,王一洲..伸筋易骨矫形手法对兔膝关节软骨细胞代谢及 Sox9表达的影响[J].天津中医药,2016,(2):96-99,4.基金项目
国家自然科学基金资助项目（81473795）。 （）
